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flow panel reactive antibody flowpra single ag beads  (Thermo Fisher)


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    Thermo Fisher flow panel reactive antibody flowpra single ag beads
    (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA <t>FlowPRA</t> beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.
    Flow Panel Reactive Antibody Flowpra Single Ag Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+panel+reactive+antibody+flowpra+single+ag+beads/pmc06483008-120-7-5
    Average 90 stars, based on 1 article reviews
    flow panel reactive antibody flowpra single ag beads - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells"

    Article Title: Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells

    Journal: JCI Insight

    doi: 10.1172/jci.insight.123672

    (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA FlowPRA beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.
    Figure Legend Snippet: (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA FlowPRA beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.

    Techniques Used: Incubation, Binding Assay, Flow Cytometry, Negative Control

    Related Articles

    Labeling:

    Article Title: Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells
    Article Snippet: .. Specifically, we hypothesized that the One Lambda Flow Panel Reactive Antibody (FlowPRA) single Ag beads previously developed to measure serum alloantibody titers ( 35 ), which consist of fluorescently labeled beads coupled to single HLA Ags, could be adapted to measure alloAg-directed CAR Treg specificity. ..



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    flow panel reactive antibody flowpra single ag beads  (Thermo Fisher)
    90
    Thermo Fisher flow panel reactive antibody flowpra single ag beads
    (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA <t>FlowPRA</t> beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.
    Flow Panel Reactive Antibody Flowpra Single Ag Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+panel+reactive+antibody+flowpra+single+ag+beads/pmc06483008-120-7-5
    Average 90 stars, based on 1 article reviews
    flow panel reactive antibody flowpra single ag beads - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA FlowPRA beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.

    Journal: JCI Insight

    Article Title: Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells

    doi: 10.1172/jci.insight.123672

    Figure Lengend Snippet: (A) Schematic diagram of experimental setup and gating strategy for the FlowPRT cell assay. ΔNGFR or CAR Tregs were incubated with a cocktail of single HLA FlowPRA beads for 30 minutes, and bead-CAR Treg interactions were quantified as the loss of beads in a bead singlet gate based on FSC/SSC profile. (B) Binding to HLA-A*02:01–coated beads for each m/hA2-CAR Treg relative to binding of a ΔNGFR Treg control. Statistical significance determined by 1-way ANOVA and Holm-Šídák post hoc test comparing with mA2-CAR; mean ± SEM; **P < 0.01. (C and D) Correlation between the mean of HLA-A*02:01 binding measured by the FlowPRT cell assay and either (C) HLA-A*02:01 tetramer MFI evaluated by flow cytometry or (D) increase in the proportion of CD69+ cells 16 hours after coculture with HLA-A*02:01 versus negative control HLA-A*24:01 K562 cells. (E) Percent binding of each m/hA2-CAR Treg to the indicated HLA-A alleles after normalization to an ΔNGFR Treg control from the same donor. Dotted line represents 2 SDs from the mean of the bead-only control. For a summary of statistical results in E, see Supplemental Table 1. n = 3–6 from at least 3 independent experiments.

    Article Snippet: Specifically, we hypothesized that the One Lambda Flow Panel Reactive Antibody (FlowPRA) single Ag beads previously developed to measure serum alloantibody titers ( 35 ), which consist of fluorescently labeled beads coupled to single HLA Ags, could be adapted to measure alloAg-directed CAR Treg specificity.

    Techniques: Incubation, Binding Assay, Flow Cytometry, Negative Control